Includes a theory section, mass spectral analysis, and report preparation. <>
After-Hours Necropsy Emergency | See the Necropsy Service for more details. ET, (517) 353-2296 Saturday, Microbiology Only, 9:00 - 11:00 a.m. The function of the Toxicology Section at the MSU VDL is to analyze biological, food/feed, water, and environmental samples to detect the presence of toxic compounds and determine whether poisons are responsible for animal illness or death. 1. 4 0 obj
If detergent is necessary for your sample workup, additional cleaning steps will need to be implemented to remove the detergent before analysis. American Association of Veterinary Laboratory Diagnosticians Appropriate sample submission can be discussed during the initial project consultation. Samples should be clearly labeled with their contents, organism of origin, treatment condition, buffers, volumes, and concentrations if known. For global protein identification, we ask that you submit at least 10 µg of total protein per sample. �D⮂��AoMVB�ׅJy���[�HeZ�j���9���%B9��- �vl�'�fV���A�{]M2��B�g��E,�,��T�uP:�Ăe �"-]�P��8�m��L�"�.d�f�)�q��� � H�Ů This is a six-hour hands-on training on protein sample preparation for tandem mass spectrometry analysis. x��\Yo�H~7��Џ�"f��l'sƙ̌�dd���H�"R�d�VUS��fw˞`��e�Ů��n���L�&c�)S�1��T�L�4c�������{���|������z��^���;�\��$�L0!S��H�.����E�5��9>����%��Kv�8>��#���������$M79�I�&e�,���\x���D����D��MNO~��2�.&�$U;9Q����~�-'�f�?�F~=>��lK)�g�'�|5��bm=9ɒ(�e�5�?�����ˏ) ��nP�����w$ՌM�Hn�sCW�M�''�.�����9~��{�ƲЯB7ޒ�@y2%u���뫊��|�鬒�sOE��?0OUv+��ZYsݐp ��=�_A00)�w����3�p�?HYS��Tk|����kn@R����67x]�+�Q�>���\��_^0D ��M��A�0�`��A�_���І��#ܛ2Ky��1B�Z{��C��HQ�R˽�����H���϶^"�Yw[M��O���x�z�����~�Dc���|�ǚ2���=������H����%O[J���Wb't^���?��߿��-���Z���� -��6�a�0\��s�>��R�\�Ϯ&����xe�,�/u��DE~��{��qo+zD�t���&'C���I$�� Instrument time is defined as 1 unit = 1 hr. Please call 517.353.1683 if you have any questions about Toxicology testing. In order to prevent protein degradation and modification, all sample manipulations should be performed on ice. 550-570 Problems: none 1. However, because of our expertise and the sophisticated equipment at our disposal, we can serve clients that need help with unusual cases. In such cases, we will work with you to develop the test method and to validate that method. Used when the experiment requires validation of the samples condition or standardization. Includes a theory section, in-gel and in-solution digestion experiments. Lysates should be stored in protease inhibitor buffer (potentially with phosphatase inhibitor). stream
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1 unit = 1 sample, Includes sample preparation and protein digestion for tandem mass spectrometry. Qualitative and quantitative composition of complex samples. If not frozen, samples may be kept on regular ice. This is a six-hour hands-on training on protein sample preparation for tandem mass spectrometry analysis. Integrated Mass Spectrometry Unit. 1 unit = up to 10 samples, One sample per gel. 1 unit = 1 sample, Instrument time is defined as 1 unit = 2 hr. Tests on food producing animals play a role in food safety. Please call the lab at 517.353.1683 to inquire about a consultative workup, hourly rates, or to determine if we can assist you with a custom assay. The Toxicology Laboratory normally runs routine samples of clinical or diagnostic nature. Use low retention Eppendorf tubes. We advise you to first clean Eppendorf tubes with 70% EtOH. <>>>
Samples should be shipped on dry ice and/or with frozen gel packs. Do not microwave your gel or scan with an overhead projector foil. It is impossible to completely prevent human keratin contamination of your samples. The MSU VDL Toxicology Section is equipped to test for an almost infinite range of toxic substances and drugs. To reserve a spot on the MS calendar, go to googleapps.msu.edu and use Google calendar. 1 unit = 1 hr, Database analysis of raw MS spectra, deconvolution, preparation of reports and figures. ET. 4. We must be informed of any detergents in your samples before submission. Digestion utilizes a combination of Trypsin and LysC unless otherwise requested. It is worthwhile to optimize their separation before submission. Typically, Quantitative Proteomics experiments will take 6 hours of instrument time per sample (i.e. The RTSF Mass Spectrometry and Metabolomics Core is an ‘open access’ resource where MSU researchers and Core staff perform chemical analyses for small molecules and intact macromolecules. 2. The three essential functions of a mass spectrometer, and the associated components, are: Please use either Coomassie G-250 or SilverQuest Silver Stain for staining gels. Mass Spectrometry & Metabolomics Core Facility 603 Wilson Rd., Room 11 Biochemistry Building East Lansing, MI 48824 517-353-0612 RTSF.MassSpec@msu.edu Please consult our test catalog for a comprehensive list of toxicants we test for. However, some general guidelines are provided. 1 unit = 1 sample. Analyses performed here typically involve compound identification, quantification, and … Never seal a package that contains dry ice. Avoid buffers containing detergents (SDS, SDC, SLS, Triton, Tween, NP-40, IPEGAL, PEG, etc.). Alternativity, a printout detailing sample information may be submitted with samples. Irving Vega, Ph.D. Facility personnel will be available to discuss experimental design, sample preparation, data mining, and validation. <>
Monday-Friday: 7:00 a.m. – 10:00 p.m. %����
BLD 872 - Clinical Mass Spectrometry Laboratory (2 credits) This one week intensive laboratory course is offered during the summer semester here on the campus of Michigan State University. Cut gels bands to include only to the region of interest. For complete information about Toxicology tests, specimen requirements, or submittal procedures please see our catalog of available tests.